Cell Line Origin
Mouse / Human Hybrid, NIH-3T3 transfectant
Cell Line Description
The cell line DF has been derived by transfecting NIH-3T3 with human chromosome 7 DNA. It was originally established for the analysis of the cystic fibrosis locus and contains a transgenomic size of 3-4 Mb. Chromosomal fragments detected were 7q22-31.3 (met oncogene) and 7q22-7cen.
Application
Analysis of human chromosome 7 transfectants.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-6 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5%CO2; 37°C.
Other Notes
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biological source: Human x Mouse Hybridoma unknown/unspecified. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 86072802-1VL