General description
A cell-permeable triazolopyrimidine compound that potently inhibits malaria parasite Plasmodium falciparum dihydroorotate dehydrogenase- (PfDHODH-) catalyzed electron transfer from FMNH2 to Coenzyme Q10 (CoQ or ubiquinone) in a potent (IC50 = 47 nM), CoQ-competitive (Ki = 15 nM), and species-specific manner, without affecting PfDHODH-catalyzed electron transfer from DHO to FMN or the enzymatic activity of human DHODH (<5% inhibition at 100 µM). Reported to inhibit the proliferation of P. falciparum parasites (EC50 = 79 nM against 3D7 strain) in vitro, including the drug-resistant strain Dd2 (EC50 = 140 nM), while displaying no inhibitory potency against murine lymphocytic leukemia cell line L1210 even at concentrations as high as 10 µM. DSM1 is not recommended for in vivo use due to reduced plasma exposure upon repeat dosing in mice.
A cell-permeable triazolopyrimidine compound that potently inhibits malaria parasite Plasmodium falciparum dihydroorotate dehydrogenase- (PfDHODH-) catalyzed electron transfer from FMNH2 to Coenzyme Q10 (CoQ or ubiquinone) in a potent (IC50 = 47 nM), CoQ-competitive (Ki = 15 nM), and species-specific manner, without affecting PfDHODH-catalyzed electron transfer from DHO to FMN or the enzymatic activity of human DHODH (<5% inhibition at 100 µM). Reported to inhibit the proliferation of P. falciparum parasites (EC50 = 79 nM against 3D7 strain) in vitro, including the drug-resistant strain Dd2 (EC50 = 140 nM), while displaying no inhibitory potency against murine lymphocytic leukemia cell line L1210 even at concentrations as high as 10 µM. DSM1 is not recommended for in vivo use due to reduced plasma exposure upon repeat dosing in mice.
Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
DHODH Inhibitor, DSM1, is a highly potent, competitive, tight-binding inhibitor of Plasmodium falciparum dihydroorotate dehydrogenase (IC₅₀ = 47 nM; Ki = 15 nM).
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Pf*DHODH
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Guler, J.L., et al. 2013. PLoS Pathog.9, e1003375.
Ke, H., et al. 2011. Eukaryot. Cell.10, 1053.
Ganesan, S.M., et al. 2011. Mol. Biochem. Parasitol.177, 29.
Booker, M.L., et al. 2010. J. Biol. Chem.285, 33054.
Gujjar, R., et al. 2009. J. Med. Chem.52, 1864.
Phillips, M.A., et al. 2008. J. Med. Chem.51, 3649.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Molecular Weight: 275.31. Empirical Formula: C16H13N5. Assay: ≥. 97% (HPLC). Quality Level: 100. form: solid. potency: 15 . nM Ki. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, protect from light. color: off-white. solubility: DMSO: 50 . mg/mL. storage temp.: 2-8°C. SMILES string: CC1: NC2: NC: NN2C(: C1)NC3: CC4: CC: CC: C4C: C3. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51182012
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 5333040001