General description
Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.
Application
Diethyl pyrocarbonate was used as:
- nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissues
- an antimicrobial additive
- ribonuclease inhibitor
- histidine residue modifier
- reagent for conversion of imines to carbamates
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
Biochem/physiol Actions
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
- UPC:
- 51303101
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 159220-25G