General description
Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.
Application
Digoxigenin-11-UTP has been used for labeling RNA probes in:
- in situ hybridization
- in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
- fluorescence in situ hybridization (FISH)
Biochem/physiol Actions
Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.
Quality
Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4
Other Notes
For life science research only. Not for use in diagnostic procedures.
- UPC:
- 41115812
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 11209256910
