General description
Native, DPP IV purified from porcine kidney (renal brush border of the proximal tubule). A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. Cleaves Xaa-Pro dipeptides from the N-terminus of oligo and polypeptides. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, T cell activation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. MW: ~210000-260000 (unreduced).
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmole 7-(Gly-Pro)-amino-4-methylcoumarinamide per min at 37°C, pH 8.5.
Physical form
In 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.
Reconstitution
Dilute only the amount of enzyme needed for each assay. Upon thawing, do not re-freeze, store in the refrigerator (4°C).
Other Notes
Bar, J., et al. 2003. Biol. Chem.384, 1553.
Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA100, 5063.
Ikehara, Y., et al. 1994. Methods. Enzymol.244, 215.
David, F., et al. 1993. J. Biol. Chem.268, 17247.
Thomsen, P.D., et al. 1993. Mamm. Genome4, 604.
Misumi, Y., et al. 1992. Biochim. Biophys. Acta.1131, 333.
Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler372, 213.
Checler, F., et al. 1985. J. Neurochem.45, 1509.
Imai, K., et al. 1983. J. Biochem.93, 431.
Kato, T., et al. 1979. Experientia.35, 409.
Kojima, K., et al. 1979. Anal. Biochem.100, 43.
Kato, T., et al. 1978. Biochem. Med.19, 351.
Kenny, A.J., et al. 1976. Biochem. J.157, 169.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 12352202
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 317640-250MIU
- Product Size:
- 250MIU
