Diptheria Toxin CRM197 Mutant Vaccine Carrier Protein, 1mg

Description: EcoCRM™ is CRM₁₉₇ produced as a soluble intracellular protein in E. coli. CRM₁₉₇ is genetically detoxified diphtheria toxin. Specifically, it has a single amino acid substitution (glycine52 to glutamic acid), which causes the ADPribosyltransferase activity to be lost. CRM197 is widely used as the carrier protein for conjugate vaccines. EcoCRM™ is expressed as a recombinant protein in Escherichia coli. Supplied by: Fina Biosolutions LLC Size: 1 mg of protein is supplied at a concentration of 1.0 mg/mL (by absorbance at 280 nm) in 1X PBS, pH 7.6, 10% glycerol. The theoretical molecular weight of the protein is 58.4 kDa. Purity: >95% by HPLC OD₂₆₀/OD₂₈₀ = 0.57 (< 1.0, the protein is free from nucleic acid contaminant) Endotoxin: 0.16 EU/µg Relevance: CRM₁₉₇ is widely used as the carrier protein for conjugate vaccines. Vaccines for Haemophilus influenzae b, meningococcal disease and Streptococcus pneumoniae use CRM197. Unlike toxoids, CRM₁₉₇ has its full complement of lysines available for conjugation. It is also thought to cause less interference in multivalent vaccines. EcoCRM™ is stable in PBS. Storage: -80°C, avoid repeated freeze/thaws Intended for research use only, not for human, therapeutic, or diagnostic applications.