DiR' [DiIC18(7)] (C09-0836-723)

Product IntroductionMaximum absorption wavelength/emission wavelength: 748/780 nmRecommended filter setting: 710 ex/760 emFigure 1. T-cells isolated from the spleen were fluorescently stained with DiR and i.v. injected (5x106 cells/mouse) into a Nu/Nu mouse. Images above taken 24hrs post injection with IVIS Spectrum  show cells homing to the spleen.DiR dye is a lipophilic near-infrared cyanine fluorescent dye that can be used to dye cell membranes and other fat-soluble biological structures. A long chain of 18 carbons is inserted into the cell membrane to stain cells, while the transfer of dye between cells is negligible. DiR emits near-infrared fluorescence that can penetrate cells and tissues and is used for tracking in in vivo imaging.DIR generally performs fluorescent staining of primary cells and can observe the distribution of in vivo imaging, (For example, the following cell embryonic stem cells, bone marrow derived stem cells, adipose derived stem cells, lymphocytes and erythrocytes),Figure 2. DiR stock was prepared by dissolving 25 mg in 3 mL ethanol. Working solution of 320 µg/mL was prepared by diluting 199 µL of stock solution in 5 mL PBS. T-cells isolated from the spleen were incubated with 320 µg/mL DiR. After 30 min incubation, cells were spun down for 3 min at 1000 rpm at 4 ºC resulting in a blue pellet. Cells were washed twice in PBS and injected intravenously (5 x 106 cells/mouse). Control group was injected with 5 x 106 cells/mouse in PBS. Mice were imaged with IVIS Spectrum  at 10 min, 1hr, 6hr and 24 hrs post injection. Ideal filter set for DiR imaging is 710 nm excitation and 760 nm emission. Mice were imaged dorsally as well as ventrally at all time points. Brain, bones, spleen, liver, lungs and kidneys were harvested for ex vivo imaging 24 hrs post injection.Non-invasive in vivo imaging showed the homing process of injected T cells to the liver and spleen in real time, which was confirmed by ex vivo imaging.ReferencesKalchenko et al., Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. Journal of Biomedical optics, September/October 2006, Vol 11(5). Specification: ≥95.0% Molecular Formula: C63H101IN2 Molecular Weight: 1013.41 PubChem CID: 74405886 Isomeric SMILES: CCCCCCCCCCCCCCCCCCN1C2=CC=CC=C2C(C1=CC=CC=CC=CC3=[N+](C4=CC=CC=C4C3(C)C)CCCCCCCCCCCCCCCCCC)(C)C.[I-] Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/D131031-SCI_c66b4f7c066d238225489367243e5683.pdf