General description
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Features and Benefits
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Recommended products
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Discovery is a registered trademark of Sigma-Aldrich Co. LLC
material: stainless steel column. Quality Level: 100. Agency: suitable for USP L43. product line: Discovery®. . feature: endcapped. manufacturer/tradename: Discovery®. . packaging: 1 ea of . extent of labeling: 12% Carbon loading. parameter: ≤. 70 °C temp. range, 400 bar pressure (5801 psi). technique(s): HPLC: suitable, LC/MS: suitable. L × I.D.: 5 . cm × 2.1 . mm . surface area: 300 . m2/g . surface coverage: 4 . μ. mol/m2. impurities: <. 10 . ppm metals. matrix: silica gel, high purity, spherical particle platform, fully porous particle. matrix active group: PFP (pentafluorophenyl) phase. particle size: 3 . μ. m. pore size: 120 . Ã…. operating pH range: 2-8. application(s): food and beverages. separation technique: reversed phase.- UPC:
- 41115709
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 567500-U