General description
Dithiothreitol (DTT), a thiol group protectant, is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.
Application
- in the treatment of intestinal explants
- in homogenate preparation of gill cells for enzymatic assays
- in protein electrophoresis for western blotting
- in transglutaminase activity
- in sample buffer for SDS-PAGE
- in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing
- as a component of protein extraction buffer for cell lysis
Biochem/physiol Actions
Features and Benefits
- BioUltra grade - 1M DTT solution, suitable for molecular biology
- Free from DNases, RNases, phosphatases, and proteases
- UPC:
- 51473010
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 43816-50ML