Cell Line Origin
Human colon adenocarcinoma
Cell Line Description
Derived from human colorectal adenocarcinoma. The cells have been used in the study of polar solvents on cell characteristics.The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.
Application
DLD-1 cell line has been used to express shRNA oligos directed against coronin 2A (CRN5) and to study the role of CRN5 in tumor cell migration. It has also been used to determine the efficiency of antibody-coupled short interfering RNA (siRNA) for in vivo mRNA knockdown.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin, 0.02% EDTA.
Other Notes
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biological source: human colon. growth mode: Adherent. karyotype: 2n : 46, pseudodiploid. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51111622
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90102540-1VL
