General description
DLD1 Cells BAX -/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
Application
Media Renewal changes two to three times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vail cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:10
The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164)
Features and Benefits
These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 19q13.3-q13.4
Quality
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
- UPC:
- 41105330
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- CLLS1001-1SET