DNA MW-MARKER XIV

General description

Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 100 to 1500 bp and an additional band of 2642 bp

Application

DNA Molecular Weight Marker XIV (100 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest or RT-PCR, following separation on agarose gels. The DNA molecular weight marker can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder) for precise size determination.
Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,³²P-dTTP or ³²P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.

DNA Molecular Weight Marker XIV has been used in gel electrophoresis.

Sequence

The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, and an additional band of 2642 bp. The 500-bp and 1000-bp banding pattern is two to three times brighter.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.To make them easy to identify, the 500 and 1,000 bp bands are two to three times as bright as the other bands in the ladder.

Unit Definition

50 μg = 1A₂₆₀ unit

Physical form

Ready-to-use solution, 250 μg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).

Other Notes

For life science research only. Not for use in diagnostic procedures.