DNASE I LYO. 100 MG

General description

DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin.

Application

DNase I has been used for tissue dissociation in conjunction with collagenases.

Biochem/physiol Actions

During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.

Features and Benefits

• Lyophilized
• Filtered through 0.2 μm pore size membrane before lyophilization
• Glycoprotein and double-strand-specific endonuclease

Unit Definition

One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.

Preparation Note

Activator: Bivalent metal ions (Ca²⁺, Mg²⁺)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg²⁺.

Reconstitution

Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.

Other Notes

For life science research only. Not for use in diagnostic procedures.