General description
RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
form: liquid. manufacturer/tradename: Novagen®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. shipped in: wet ice. storage temp.: −. 20°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41121600
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 69182-3
