DNase Test HiVeg Agar Base without DNA, 500G

DNase test HiVeg™ agar base w/o DNA is recommended with the addition of DNA it is used for detection of deoxyribonuclease activity of bacteria and fungi, especially Staphylococci. Tryptone and soya peptone provides essential nutrients. DNase activity is detected by flooding the surface of the medium with 1 N HCl and observing for clear zones around the colonies on the medium (with added DNA and mannitol and no bromothymol blue). In the absence of DNase activity, cloudy precipitate is formed due to reaction of HCl with nucleic acids. When bromothymol blue is used, yellow zones are formed. Further confirmatory tests for the identification should be carried out. Serratia marcescens positive reaction, change in color from green to yellow around the growth. It is prepared by replacing animal peptones with vegetable defined peptones to avoid BSE/TSE/GMO risk. Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis Appearance of powder: Light yellow colored, may have slightly greenish tinge, homogeneous, free flowing powder Gelling: Firm, comparable with 1.5% Agar gel Color and Clarity: Light amber colored, clear to slightly opalescent gel forms in petri plates. With added 2 grams of DNA, 0.025 grams of Bromothymol blue and 10 grams of mannitol, the gel formed in petri plates is yellowish green colored Reaction: Reaction of 4.0% w/v aqueous solution is pH 7.3 ±0.2 at 25 deg C