Cell Line Origin
Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147
Cell Line Description
Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.
Culture Medium
DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.
Other Notes
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biological source: mouse unknown/unspecified. growth mode: Suspension. karyotype: Not specified. morphology: Not specified. products: Not specified. receptors: Not specified. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85082301-1VL
