DRBC (DICHLORAN-ROSE BENGAL CHLORAMPHENICOL) AGAR ACC. ISO 21527 AND FDA-BAM

General description

GranuCult^® prime Dichloran-rose bengal chloramphenicol (DRBC) agar is a selective agar for the enumeration of food-spoiling yeasts and molds. DRBC is a modification of Rose-Bengal Chloramphenicol Agar. In comparison to RBC, the medium contains Dichloran (0.002 g/L), the pH is lowered to 5.6 and the Rose-Bengal concentration is cut in half (0.025 g/L). This results in an increased inhibition of bacteria and yeasts. The inclusion of dichloran serves to inhibit the rapid spreading of mucoraceous fungi and restricts colony sizes of other genera, easing the colony count.

Application

GranuCult^® prime Dichloran-rose bengal chloramphenicol agar is suitable for the enumeration of food-spoiling yeasts and molds.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear to slightly opalescent
Appearance (colour): pink
pH-value (25 °C): 5.4 - 5.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Colony count (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): ≥ 50 %
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 50 %
Inoculum on reference medium (Mucor racemus ATCC 42647 (WDCM00181)):
Colony count (Mucor racemus ATCC 42647 (WDCM00181)):
Recovery on test medium (Mucor racemus ATCC 42647 (WDCM00181)): ≥ 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth Escherichia coliATCC 25922 (WDCM 00013): no growth
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): no growth
Incubation: 5 days at 25 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: SABOURAUD dextrose agar

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany