Cell Line Origin
Mouse mammary, drug-resistant
Cell Line Description
The drug resistant cell line EMT6/AR10.0 was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by continuous growth in increasing concentrations of doxorubicin. It was reported to show reduced intracellular drug accumulation and hyperexpression of P-glycoprotein. It is recommended to culture the cells without drug after resuscitation until the first passage.
Application
Drug-resistance studies
Culture Medium
MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA)+ 10.0μg/ml doxorubicin + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5%CO2; 37ºC; resuscitate in drug-free media, add doxorubicin after first passage
Other Notes
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biological source: mouse unknown/unspecified. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51343105
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 96042326-1VL
