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drug resistant variant of nci-h69 human (c15-1314-518)

DRUG RESISTANT VARIANT OF NCI-H69 HUMAN (C15-1314-518)

Sigma Aldrich

Catalog No.
C15-1314-518
Manufacturer No.
96042329-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI
Price: $1,835.55
List Price: $2,039.50

Cell Line Origin Human Caucasian small cell lung carcinoma, drug-resistant Cell Line Description NCI-H69/LX4 has been established by exposure of the parent line, H69 (Sigma Catalogue number. 91091802) to doxorubicin, also known as adriamycin.

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Cell Line Origin

Human Caucasian small cell lung carcinoma, drug-resistant

Cell Line Description

NCI-H69/LX4 has been established by exposure of the parent line, H69 (Sigma Catalogue number. 91091802) to doxorubicin, also known as adriamycin. The line hyperexpresses P-glycoprotein and demonstrates a multidrug resistant drug accumulation deficit. It is recommended to culture the cells without drug after resuscitation until the first passage.

Application

Analysis of acquired drug-resistance, study of small cell lung cancer

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Culture Medium

RPMI + 2mM Glutamine + 0.4μg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) by repeated pipetting of aggregates or scraping; 5% CO2; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: human lung. growth mode: Aggregates in suspension. karyotype: Not specified. morphology: Not specified. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
51142318
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
96042329-1VL

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