Dubos HiVeg Broth Base is for preparation of liquid medium for rapid cultivation of pure cultures of Mycobacterium tuberculosis and related microorganisms. Pack size is 5KG

This medium is prepared by using HiVeg hydrolysate in place of Casein enzymic hydrolysate which makes the medium free from BSE/TSE risks. Dubos HiVeg Broth Base is the modification of original medium of Dubos and Davis (1) and Dubos and Middlebrook (2). Dubos HiVeg Broth Base can be used for the preparation of variety of media for the rapid cultivation of Mycobacterium tuberculosis. This medium is valuable for research on antituberculosis drugs, for preparation of inocula for animal studies, etc. Dubos HiVeg Broth Base can be helpful in diagnostic procedures in which specimens such as spinal or pleural fluids may be expected to yield pure cultures. Dubos HiVeg Broth Base contains HiVeg hydrolysate and L-Asparagine as source of nutrients, inorganic salts provide ions required for the metabolism. Glycerol serves as essential fatty acids for the multiplication of Mycobacteria and the media has strong buffering system (4). Polysorbate 80 (Tween 80) acts as surfactant facilitating the even distribution of two phases and dispersion of Mycobacterial cells, thereby enhancing their growth. Dubos HiVeg Broth Base enriched with serum will generally initiate growth from smaller inocula and yield more luxuriant growth than the basal medium enriched with albumin V. Growth is generally more granular with the serum enrichment, while it is more diffused with albumin enrichment. Maximum care should be taken while handling Mycobacterial cultures as they are highly infectious. Directions: Suspend 1.3 grams in 180 ml distilled water containing 10 ml Glycerol. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 20 ml sterile bovine albumin V or sterile serum or 1 vial of sterile Albumin Glucose Supplement (FD201) to each 180 ml of broth base.