Dulbecco's Modified Eagle Medium (DMEM), with 4.5gms Glucose per liter, 1mM Sodium pyruvate, L-Glutamine, 1.5gms per liter of Sodium bicarbonate, 5x500 mL

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000 mgs/L of glucose and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4500mgs/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. C985E63, C985E64 and C985E65 is Dulbecco's Modified Eagle Medium with 4.5gms glucose per litre, L-glutamine, 1.5gms per litre sodium bicarbonate and 1mM sodium pyruvate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. Storage and Shelf Life: Store at 2-8 °C away from bright light. Shelf life is 12 months. Use before expiry date given on the product label.