DUOLINK FLOWPLA MOUSE/RABBIT STARTER KIT - VIOLET

Specificity

Violet Fluorescence Detection Reagents
Use appropriate laser for λ_ex 390 nm excitation
Use appropriate filter for λ_em 476 nm emission.

Application Note

Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended.

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Application

Based on proximity ligation assay (PLA), the Duolink^® PLA technology allows for endogenous detection of protein interactions, post-translational modifications, and protein expression levels at the single molecule level in fixed cells.

Duolink^® flowPLA Starter Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink^® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest and the Duolink^® flowPLA starter Kit. The flowPLA Starter Kits are available with 3 different fluorophores: Violet, Green, and FarRed. The flowPLA starter Kits contain all the necessary reagents reagents for 100 Duolink^® PLA reactions, including a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS) and all the reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, (rabbit and mouse-raised) primary antibodies, and corresponding PLA Probes.

Follow the Duolink^® PLA Flow Cytometry Protocol to use this product.

Visit our Duolink^® PLA Flow Cytometry page on how to run a Duolink^® flow experiment, applications, troubleshooting, and more.

Features and Benefits

• Analyze protein protein interactions with flow cytometry readout
• Analyze cell populations with Proximity Ligation Assay
• Increased sensitivity due to rolling circle amplification for low abundant targets
• No overexpression or genetic manipulation required
• Relative quantification possible
• Works with any flow cytometer instrumentation
• Easy to follow flexible protocol
• Publication-ready results

Components

Duolink^® flowPLA Detection Kit - Violet DUO94005-40TST
Duolink^® In Situ PLA^® Probe Anti-Mouse MINUS DUO92004-40TST
Duolink^® In Situ PLA^® Probe Anti-Rabbit PLUS DUO92002-40TST
Duolink^® In Situ Wash Buffer, Brightfield (DUO82047-4L)

See datasheet for more information.

Legal Information

Duolink is a registered trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC

Duolink is a registered trademark of Sigma-Aldrich Co. LLC

PLA is a registered trademark of Sigma-Aldrich Co. LLC

Plexiglas is a registered trademark of Arkema