General description
Anti-Phosphotyrosine antibody, clone 4G10® with the Duolink PLA PLUS oligonucleotide. This antibody detects tyrosine phosphorylated proteins in all species. Protein target specificity can be obtained by pairing with an antibody specific to a protein of interest in a Duolink PLA experiment. This product must be used in combination with a MINUS PLA probe of a different species or another directly-conjugated primary antibody with a MINUS PLA oligo. The Anti-mouse PLA probe PLUS (DUO92001) is not necessary when using this product.
Specificity
This directly-conjugated primary antibody PLA probe recognizes tyrosine-phosphorylated proteins from all species.
Application
Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
Application Note
Protein phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Anti-phosTyr antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosine phosphorylation of the protein of interest. This process is time-consuming and cannot provide subcellular localization.
Anti-phosTyr PLA probes are used to detect the phosphorylation of tyrosine residues in proteins. Protein target specificity can be obtained by pairing with an antibody specific to a protein of interest in a Duolink® PLA® experiment. This product is particularly useful when site-specific anti-phosphorylation antibodies are not available. A MINUS PLA probe of a different species or another directly-conjugated primary antibody with a MINUS PLA oligo must be used in combination with this product. The Anti-mouse PLA probe PLUS (DUO92001) is not necessary when using this product. See our Product Selection Guide for more information.
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
Duolink is a registered trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC
- UPC:
- 51314369
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- DUO87009-50UG
