DUTP PCR GRADE 25 UMOL

General description

dUTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Application

dUTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Avoid carryover contamination between PCRs to eliminate a source of false positives.

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physical form

solution of dUTP water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP (10 mM, each), dUTP (30 mM); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP (each) and 30 μl dUTP to 40 μl Water,PCR grade).

Other Notes

For general laboratory use.

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).