DyLight™ 633 Maleimide

Product introduction:DyLight 633 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 633 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes. fluoresces red and has physical properties comparable to other 633 dyes, including Alexa Fluor™ 633, over a broad pH range (pH 4–9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.Features of DyLight 633 Maleimide:• :High performance—:DyLight 633 shows brighter fluorescence than Alexa Fluor 633:• :Specific—:maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH):• :Efficient labeling methods—:well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling:• :Optimized antibody labeling procedure—:complete protocol for IgG reduction and labeling and calculating the labeling efficiency:Applications:• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay:• Target macromolecule labeling for :in vitro: and :in vivo: fluorescent detection strategies:DyLight 633 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5–7.5. Learn more aboutmaleimide chemistry.