Cell Line Origin
Human Caucasian infectious mononucleosis lymphocytes
Cell Line Description
Established by seeding peripheral blood lymphocytes from 20 year old female, 2 months after onset of infectious mononucleosis. EBV transformed B-lymphoblastoid line. The cells should be handled under laboratory containment level 2. Cells are particularly resistant to poliomyelitis and vesicular stomatis, viruses similar to other cell lines carrying EB virus.
Application
Transformation and virus studies: EBV
Culture Medium
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 4-5x100,000 cells/ml; 5% CO2; 37°C. Subculture every 8 days by centrifugation. Maximun of 1-2 x 1,000,000 viable cells/ml is obtainable.
Other Notes
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biological source: human blood. growth mode: Suspension. karyotype: 2n : 46. morphology: Lymphoblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51342501
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90121307-1VL