E11 SNAKEHEAD WHOLE FRY TISSUE

Cell Line Origin

Snakehead fish, whole fry tissue

Cell Line Description

E11 is a clone of the cell line SSN-1 and is persistantly infected with a C-type retrovirus (SnRV). It is susceptible to piscine nodavirus strains belonging to different genotypes (SJNNV, RGNNV, TPNNV and BFNNV - striped jack, red spotted grouper, tiger puffer and barfin flounder nervous necosis viruses repectively). E11 is highly permissive to nodavirus infection and production. An advantage of E11, over the parental line SSN-1, is its steady, faster growth to confluency and longer stability in monolayer cultures for 2 weeks at 25 °C or 4 weeks at 20 °C and its reproducible, clear CPE characterised by cytoplasmic vacuole formation followed by intensive disintegration.

Culture Medium

L15 + 5% Fetal Bovine Serum (FBS)+ 2 mM Glutamine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4, ie., seeding at 3 x10,000 cells/cm² using 0.05% trypsin/EDTA. No CO₂. Culture at 25 °C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Professor Toshihiro Nakai, PhD Laboratory of Fish Pathology Graduate School of Biosphere Science Hiroshima University 1-4-4 Kagamiyama, Higashi-Hiroshima Hiroshima 739-8528, Japan

For complete product information, please see ECACC

Disclaimer

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

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