E235 (C007B-353448)

E235 is an expression regulator of activates transcription factor 4 ( ATF4 ). E235 reduces cell viability by activating integrated stress response (ISR) and DNA damage response signals. E235 has anti-proliferative activity and can be used for tumor researchIn VitroE235 (1 µM; 2, 4, 8, 16 or 24 h) down-regulates the level of XBP-1s mRNA in HT1080 cells with time dependent manner. E235 (0.1-10 µM; 4 d or 5 d) has anti-proliferative activity on HT1080, RPMI-8226, B16F10, 4T1, HT1080 shNT and HT1080 shATF4 cells. E235 (0, 1, 5 and 10 µM; 4 h) increases the expression of p53 with dose-dependent manner in AG1522 cells. E235 (1 µM; 2 h) increases the expression of p-Chk2 in AG1522 cells. E235 (0, 1, 5 and 10 µM; 2 h) increases the expression of p-p53 in AG1522 and HT1080 cells. E235 (0, 0.5, 1, 5 and 10 µM; 0.5 h) increases the expression of γ-H2AX with dose-dependent manner in HT1080 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: HT1080, B16F10 and AG1522 cells. Concentration: 0, 1, 5 and 10 µM. Incubation Time: 4 h. Result: Increased the expression of ATF4 with dose-dependent manner. Western Blot AnalysisCell Line: HT1080 cells. Concentration: 0, 0.5, 1, 5 or 10 µM. Incubation Time: 2, 4 or 8 h. Result: Increased the expression of p-elF2α with time and dose dependent manner. Cell Proliferation AssayCell Line: HT1080 or RPMI-8226 cells. Concentration: 1 µM. Incubation Time: 0, 8, 16 and 24 h. Result: Exhibited anti-proliferative activity. Cell Viability AssayCell Line: AG1522 cells. Concentration: 0, 0.1, 0.5, 1 and 10 µM. Incubation Time: 4 d. Result: Inhibited cell viability with dose-dependent manner. Cell Cycle AnalysisCell Line: HT1080 cells and B16F10 cells. Concentration: 1 µM. Incubation Time: 8, 16 and 24 h. Result: Caused cell arrest during G2/M phase.Form:SolidIC50& Target:Activates transcription factor 4, ATF4. Specification: 0.98 Molecular Formula: C28H25FN4OS Molecular Weight: 484.59