Cell Line Origin
Saltmarsh caterpillar Estigmena acrea (Subclone 4)
Cell Line Description
This cell line can be used to produce complex glycoproteins, and has been demonstrated as having more extensive N-glycosylation compared to other insect cell lines used for the expression of recombinant proteins. Ea.4 is susceptible to infection by Baculovirus, which can be used as a vector for recombinant protein synthesis.
Application
Recombinant complex glycoprotein production, Baculovirus infection.
Culture Medium
TiterHigh™ SF Insect Medium (Sigma I5408) (no supplements)
Subculture Routine
Maintain cultures between 2x100,000 and 1x1,000,000 cells/ml. Doubling time 36 hours. Grow at 26°C. Freeze in 90% HI-FBS plus 10% glycerol.
Other Notes
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Legal Information
TiterHigh is a trademark of Sigma-Aldrich Co. LLC
biological source: insect larvae. growth mode: Suspension. karyotype: Not specified. morphology: Round. products: Complex glycoproteins. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51203903
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 96073116-1VL
