General description
ECL™ Western Blotting System is the world′s most widely used and referenced chemiluminescent immunodetection system.
ECL™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
Each system contains the following reagents, sufficient for detection of 1000 cm2 membrane:
Anti-mouse HRP conjugate, 100 μl
Anti-rabbit HRP conjugate, 100 μl
ECL™ Detection Reagents 1 and 2, 62.5 mL each
Blocking agent, 5 g.
Application
ECL™ Blotting Analysis System has been used in western blotting.
Western Blotting
Features and Benefits
- Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL™ reagents.
- Optimized system provides rapid, sensitive results using simple protocols.
- Designed for use with nitrocellulose and PVDF membranes.
- High sensitivity: Up to 10 times more sensitive than colorimetric methods.
- Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
- Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
- Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
- Non-radioactive-no specialized facilities needed
- Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
ECL is a trademark of Cytiva
- UPC:
- 41105311
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- GERPN2108
