EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride), 50mg

EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product. The unstable nature of the intermediate in aqueous solutions makes 2-step coupling, however in conjunction with N-hydroxysuccinimide, a 2-step coupling is possible. EDC is ideal for peptide immobilization and hapten-carrier protein conjugation. Features EDC mediated coupling efficiency improved by N-hydroxysuccinimide Allows for 2-step coupling process Water soluble CAS Number: 25952-53-8 Molecular Formula: C₈H₁₇N₃•HCl Molecular Weight: 191.71 g/mol Spacer Arm (Å): 0 Reactive Group: Carbodiimide Reactive Toward: Primary Amine + Carboxyl Membrane Permeable: NO Water Soluble: YES Cleavable/Reversible: NO Reaction pH: 4.7 to 6.0 Applications Couple haptens or peptides to carrier proteins Conjugate carboxyl to amine groups in peptides and proteins Cross link proteins to carboxyl surfaces and beads Convert carboxyls to amine-reactive Sulfo-NHS esters Activate nanoparticlesto amine-reactive Sulfo-NHS esters DNA Labeling through 5' phosphate groups