eGFP mRNA (C09-0882-146)

Product DescriptionGFP mRNA encodes a green fluorescent protein, which can be expressed in mammalian cells. It simulates mature mRNA with 5'cap 1 Structure and 3'poly (a) tail, and is an ideal choice for studying transfection and expression using various assays.This product is a mature mRNA with 5'cap 1 Structure and 3'poly (a) tail synthesized by T7 high yield RNA transcription Kit (E131) and modified by cap 1 capping system (m082) (Fig. 1).Figure 1 Novoprotein EGFP mRNA structure. This figure is quoted from Gustavo Torres de Souza, Rafaela chitarra Rodrigues hell, J é Sica Fernanda da Silva souza Easy In Vitro Synthesis of Optimised Functioning Reporter mRNA from Common eGFP Plasmid.  Molecular Biotechnology.Product features1. cap 1 structure is more suitable for mammalian system than cap 0 structure (Arca and m7cap) and has higher transcription efficiency. The addition of poly (a) tail inhibited RNA mediated innate immune activation and increased the stability and longevity of mRNA in vivo and in vitro. Poly (a) also plays an important role in improving the efficiency of translation initiation.2. the experimental method is simple and rapid, with stable results and good reproducibility.3. high transfection efficiency.Product usage1. as a report material for gene regulation and function research.2. it is applicable to the detection of mRNA transmission, translation efficiency, cell viability and in vivo imaging.Quality ControlThere was no RNA enzyme residue, the mRNA electrophoresis band was single, and the transfection efficiency was stable.Storage temperature-Store at 20 ℃. Specification: 1mg/ml