Elastase Substrate IV, Colorimetric - Calbiochem (C15-1302-781)

General description

A specific substrate for pancreatic elastase (K_m = 100 µM; K_cat/K_m = 35,000 s^-1 M^-1 for rat pancreatic elastase; K_m = 30 µM; K_cat/K_m = 351,000 s^-1 M^-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.



Suggested protocol: see Largman, C. (1983)

A specific substrate for pancreatic elastase (k_m = 100 µM; k_cat/ K_m = 35,000 M^-1 s^-1 for rat pancreatic elastase, K_m = 30 µM; k_cat/K_m = 35,000 M^-1 s^-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
A specific substrate for pancreatic elastase

Product does not compete with ATP.

Reversible: no

km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine

Warning

Toxicity: Standard Handling (A)

Sequence

Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

Physical form

45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 562.57. Empirical Formula: C₂₅H₃₄N₆O₉. Quality Level: 100. Assay: ≥. 95% (HPLC). form: lyophilized solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles, desiccated. color: white to off-white. solubility: ethanol: 1 . mg/mL, water: soluble. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.