EMB Agar, Levine. Agar used for the isolation, enumeration and differentiation of Members of Enterobacteriaceae. Pack size is 5KG

Phenol Red Broth Medium is formulated as per Vera (2) and is recommended to determine the fermentation reaction of carbohydrates for the differentiation of microorganisms (3, 4, 5). Phenol Red Broth Medium with various added carbohydrates serves as a differential medium by aiding in differentiation of various species and genera by their ability to ferment the specific carbohydrate, with the production of acid or acid and gas (6). Phenol Red Broth Base is a complete medium without added carbohydrate, which can be used with the addition of 5-10 %, desired carbohydrate. It is used as a negative control for studying fermentations or as a base for the addition of carbohydrates. Proteose peptone and beef extract serve as sources for carbon and nitrogen. Sodium chloride is the osmotic stabilizer. Phenol red is the pH indicator, which turns yellow at acidic pH. Gas formation is seen in Durhams tubes. All of the Enterobacteriaceae grow well in this medium. In addition to producing a pH colour shift, the production of mixed acids, notably butyric acids, often results in a pungent, foul odour from the culture medium (1). Directions: Suspend 16 grams in 1000 ml distilled water,mix well. Heat if necessary to ensure complete solution. Distribute in fermentation tubes (tubes containing inverted Durham's tubes). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Aseptically add filter sterilized or autoclave sterilized carbohydrate solution to sterile basal medium.