EMB HiVeg Broth, 2.5KG

EMB HiVeg™ broth is recommended for differentiation of Gram-negative enteric bacteria from clinical & non-clinical specimens. Methylene blue and Eosin-Y inhibits gram-positive bacteria and also acts as differential indicators. Escherichia coli grows luxuriantlly on the media and shows purple with green metallic sheen since they uptake the complex methylene blue-eosin dye. Klebsiella aerogenes and Klebsiella pneumoniae shows good growth and forms pink colored colony on the media. Nonfermenters such as Shigella probably raise the pH of surrounding medium by oxidative deamination of protein, which solubilizes the methylene blue-eosin complex resulting in colorless broth. This medium is prepared by replacing animal peptones with vegetable defined peptones to avoid BSE/TSE/GMO risk. Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis Appearance of Powder: Light purple colored, homogeneous, free flowing powder may contain minute to small dark red purple particles Gelling: Firm, comparable with 1.35% Agar gel of MV317 Color and Clarity: Reddish-purple colored, opalescent gel or solution having greenish cast forms in petri plates Reaction: Reaction of 3.6% w/v aqueous solution of MV317 and 2.25% w/v aqueous solution of MV503 is pH 7.2 ± 0.2 at 25 deg C