Endo Agar, Modified, 500 g

Endo Agar was developed by Endo to differentiate gram-negative bacteria on the basis of lactose fermentation, while inhibiting gram-positive bacteria (1). Inhibition of the later was achieved without the use of bile salts as was traditionally used. Endo was successful in inhibiting gram-positive bacteria on his medium by the incorporation of sodium sulphite and basic fuchsin. The resulting Endo Agar, also known as Fuchsin Sulphite and Infusion Agar, was used to isolate the typhoid bacilli. Many modifications of this media have been done over the years. Endo Agar, modified is one of the modifications of Endo Agar. The medium contains peptic digest of animal tissue that provide nitrogen, carbon, vitamins and minerals required for bacterial growth. Sodium sulphite and basic fuchsin has inhibitory effect on gram-positive microorganisms. Lactose fermenting coliforms produce aldehyde and acid. The aldehyde in turn liberates fuchsin from the fuchsin-sulphite complex, giving rise to a red colouration of colonies. With Escherichia coli , this reaction is very pronounced as the fuchsin crystallizes, exhibiting a permanent greenish metallic lustre (fuchsin lustre) to the colonies. Storage and Shelf-life: Store below 30°C in tightly closed containers and prepared medium at 2 - 8°C and away from light to avoid photooxidation. Use before expiry date on the label. Reference: 1. Endo, 1904, Zentralbl. Bakteriol., Abt. I. Orig., 35:109.