Endo Agar with NaCl, 500 g

Endo agar w/ NaCl is used for detection and isolation of pathogenic enteric bacilli. Gram-negative bacteria can be differentiated on the basis of lactose fermentation, while inhibiting gram-positive bacteria. Sodium sulphite and basic fuchsin inhibits most of the gram-positive bacteria. Lactose fermenting Escherichia coli and coliforms produce aldehyde and acid. The aldehyde liberates fuchsin from the fuchsin-sulphite complex and colonies of lactose fermenters appear dark red. Non-lactose fermenters such as Salmonella show colorless colonies. Escherichia coli exhibits a permanent greenish metallic luster (fuchsin luster) to the colonies due to crystallization of fuchsin. Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis for Invitro Diagnostic Use Appearance: Light pink to purple homogeneous free flowing powder Gelling: Firm, comparable with 1.2% Agar gel Color and Clarity of prepared medium: Orangish pink colored, clear to slightly opalescent gel with fine precipitate forms in Petri plates Reaction: Reaction of 3.77% w/v aqueous solution at 25 deg C. pH : 7.5±0.2