Endothelial cell growth supplement from bovine pituitary

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:

• as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
• as a supplement in medium 199 for endothelial cell culture
• as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
• as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.