Cell Line Origin
Human Caucasian promyelocytic leukaemia
Cell Line Description
Eos-HL-60 is a variant of the promyelocytic cell line HL60 established by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR profiling
Application
Study of production and function of human eosinophils, analysis of differentiation.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17
Culture Medium
IDMEM + 2mM Glutamine + 0.5mM Butyric acid + 10% Foetal Bovine Serum (FBS); pH7.6; add Butyric acid to the medium just before use.
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: human blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 96100920-1VL
