Epidermal Growth Factor, human, animal component free (C007B-555829)

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (∼6 kDa), which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids.† Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion.† EGF is a member of a growth factor family, which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The location of 3 intrachain disulfide bonds in recombinant human EGF is identical to that of mouse EGF.† EGF is homologous to a sequence contained in a 19 kDa protein of vaccinia virus,† which appears to utilize the EGF receptor to gain entry into cell† EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.†Epidermal growth factor (EGF) is synthesized in Henle′s loop and the distal convoluted tubule in the kidney. It is also produced in salivary glands and duodenum.Application:Epidermal Growth Factor, human, animal component free has been used: as a supplement in in LHC-8 medium to culture liver cell lines in the fetal bovine serum (FBS)-Dulbecco′s modified essential medium (DMEM) /F12 medium for primary culture of human glioma cells as an additive in the conditional medium of normal fibroblasts (NFs) to study its effect on the migration and invasion of endometrial cancer (EC) cellsas a component in tumorsphere medium

Specification: Recombinant, for cell culture, ≥97%(SDS-PAGE), EGF, expressed in Escherichia coli

MDL Number: MFCD00132565

Molecular Formula: C270H401N73O83S7

Molecular Weight: 6.5kD

PubChem CID: 16143379

Isomeric SMILES: CC[C@H](C)[C@@H](C(=O)NCC(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC2=CNC3=CC=CC=C32)C(=O)N[C@@H](CC4=CNC5=CC=CC=C54)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O)NC(=O)[C@H](CC6=CC=C(C=C6)O)NC(=O)CNC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CS)NC(=O)[C@H](C)NC(=O)[C@H](CC7=CC=C(C=C7)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H](CC8=CC=C(C=C8)O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC9=CN=CN9)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC1=CN=CN1)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CS)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)N)N