Epiregulin, Mouse, Recombinant (C15-1302-795)

General description

Recombinant, mouse epiregulin expressed in E. coli with an N-terminal methionine. Epiregulin is shown to inhibit epithelial tumor growth, able to stimulate growth of fibroblasts and other cell types, and has been shown to be an autocrine growth factor in human epidermal keratinocytes.

Biochem/physiol Actions

ED₅₀ = 0.3-1 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Other Notes

Shirakata, Y., et al. 2000. J. Biol. Chem.275, 5748.
Shelly, M., et al. 1998. J. Biol. Chem.273, 10496.
Das, S.K., et al. 1997. Dev. Biol.190, 178.
Komurasaki, T., et al. 1997. Oncogene15, 2841.
Toyoda, H., et al. 1995. J. Biol. Chem.270, 7495.
Toyoda, H., et al. 1995. FEBS Lett.377, 403.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

recombinant: expressed in E. coli. Quality Level: 100. Assay: ≥. 97% (SDS-PAGE). form: liquid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. impurities: <. 1.0 . EU/μ. g protein Endotoxin. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.