EPOXY-ACTIVATED AGAROSE 5GR

Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligand s containing amine groups, thiol or hydroxyl groups. Epoxide-Acivated Agarose is prepared by immobilization of oxiranes such as 1,4-butanediol diglycidyl ether onto matrix. This pre-activated resin provide hydrophilic 12 atom spacer arm which enables binding of small ligand molecules such as amino acids, monosaccharide, peptides and carbohydrates. Epoxy-Activated Agarose find widespread application in affinity chromatography involving purification of antigen, antibody, lectin, glycoproteins etc. Click here to request bulk or custom sizes . Features: Resin Active group: epoxy Resin Active group density: 20-40 µM/ ml hydrated resin. Group to be coupled: -NH₂, -OH or -SH 45-165µm particle size range Spherical, highly cross-linked 6% agarose Resin spacer arm: 12 atom hydrophilic spacer arm Coupling conditions: pH 7.5-8.5 for thiol ligand s, pH 9-10 for amine ligand s and pH 11-13 for hydroxyl ligand s at 4 to 37°C depending upon ligand stability for 16 hrs pH stability: 2-13, ligand dependent 1 gm of dry resin swells to 5-6 ml. Storage: 2- 8°C, desiccated Application(s): Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. It can couple various small and big moleculeligand s via their amine, thiol or hydroxyl groups Epoxy-Activated Agarose find widespread application in affinity chromatography involving purification of antigen, antibody, lectin, glycoproteins etc