ER-Tracker Red (C007B-555784)

Precautions：ER-Tracker Red (1mM) will solidify on ice or at lower temperatures such as 4℃ and stick to the bottom, walls or lid of the centrifuge tube. It should be thawed completely in a water bath at 20-25℃ prior to use. For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.Coverslips and slides are required but not supplied. They can be ordered from .The pharmacological activity of glibenclamide may affect some functions of the ER. Variable expression of sulfonylurea receptors in some specific cells may result in non-ER-specific staining.ER-Tracker Red is suitable for staining ER in live cells, but not ER in fixed cells. When cells stained with ER-Tracker Red need to be fixed, fix cells with 4% paraformaldehyde at 37℃ for 2 minutes.ER-Tracker Red stained cells cannot be permeabilized with Triton X-100 which will result in the loss of ER-Tracker Red fluorescent staining.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1.Preparation of ER-Tracker Red working solutiona.Dilute an appropriate amount of ER-Tracker Red (1mM) in the ER-Tracker Red Dilution Buffer at a ratio of 1:1000. For example, mix 1µl of ER-Tracker Red with 1ml of ER-Tracker Red Dilution buffer to obtain the ER-Tracker Red working solution.b.Prewarm the ER-Tracker Red working solution at 37℃ before use.Note: The dilution ratio of ER-Tracker Red can be adjusted within the range of 1:1000-1:3000 based on the staining results. To minimize the staining background, a lower concentration of ER-Tracker Red is preferred as long as an acceptable staining result can be obtained.2.Fluorescent labeling of ERa.Remove the cell culture medium and wash cells grown on coverslips with an appropriate amount of solution such as Hanks' Balanced Salt Solution with Ca2+ & Mg2+ (, C0219). For staining of suspension cells, centrifuge to collect cells and refer to the staining method for adherent cells .b.Remove the wash buffer, add the ER-Tracker Red working solution pre-incubated at 37℃, and incubate at 37℃ for 15-30 min.c. Remove the ER-Tracker Red working solution and wash cells 1-2 times with cell culture medium.d.Examine cells by fluorescence microscope or confocal microscope.e.If the ER-Tracker Red stained cells need to be fixed, fix with 4% paraformaldehyde for 2 min at 37℃. After fixation, wash cells 2-3 times with appropriate wash buffer for 5 minutes each, and then counterstain cells or mount cells with an appropriate antifade mounting medium. Note: ER-Tracker Red stained cells cannot be permeabilized with Triton X-100 which will result in the loss of ER-Tracker Red fluorescent staining.Related Products：Cat. No.Product NamePack Size C1002 DAPI 5mg/ml×0.2ml C1005/C1006 DAPI staining solution 10ml/50ml C1011 Hoechst 33258 10mg C1017/C1018 Hoechst 33258 dye solution 10ml/50ml C1022 Hoechst 33342 10mg C1025/C1026 Hoechst 33342 stain 10ml/50ml C1027/C1028/C1029 Hoechst 33342 Live Cell Staining Solution (100X) 0.1ml/0.5ml/3ml C1033 Actin-Tracker Green (microfilament green fluorescent probe) 0.2ml C1036 DiI (cell membrane red fluorescent probe) 10mg C1038 DiO (cell membrane green fluorescent probe) 10mg C1039-10mg DiD (cell membrane far-infrared fluorescent probe) 10mg C1041 ER-Tracker Red (Endoplasmic Reticulum Red Fluorescent Probe) 20μl C1042S ER-Tracker Green (Endoplasmic Reticulum Green Fluorescent Probe) 20μl C1043 Golgi-Tracker Red (Golgi red fluorescent probe) 1mg C1045S Golgi-Tracker Green (Golgi green fluorescent probe) 1mg C1046 Lyso-Tracker Red (Lysosomal Red Fluorescent Probe) 50μl C1047S Lyso-Tracker Green (lysosomal green fluorescent probe) 50μl C1048 Mito-Tracker Green (mitochondrial green fluorescent probe) 50μg C1049-50µg Mito-Tracker Red CMXRos (Mitochondrial Red Fluorescent Probes) 50μg C1049-250µg Mito-Tracker Red CMXRos (Mitochondrial Red Fluorescent Probes) 50μg×5 C1050 Tubulin-Tracker Red (antibody-based microtubule red fluorescent probe) 40μl C1051S Tubulin-Tracker Green (antibody-based microtubule green fluorescent probe) 40μl C1991S Red fluorescence staining kit for cell membrane (DiI) 200-1000T C1993S Green fluorescent staining kit for cell membranes (DiO) 200-1000T C1995S Far-infrared fluorescence staining kit for cell membrane (DiD) 200-1000T C2005 JC-1 1mg C2007 Rhodamine 123 5mg