General description

Ethanolamine hydrochloride, a versatile compound in biochemistry and molecular biology, plays a pivotal role in diverse research areas. Primarily, it functions as a buffering agent, ensuring a stable pH environment crucial for enzymatic reactions, protein solubilization, and other biological processes. Its significance extends to membrane protein studies, aiding in the extraction of these proteins from cell membranes. It also finds utility in cell culture and as a reagent for biological sample preparation, simplifying nucleic acid and protein isolation, as well as facilitating the detection of proteins by western blotting and immunohistochemistry. This compound′s wide-ranging applications make it indispensable in biochemical research, contributing to various experimental procedures and advancing our understanding in fields such as molecular biology, immunology, and cellular biology.

Application

Ethanolamine hydrochloride has been used:

in overnight incubation of the tips to attach primary amine groups at the tip surface
in the preparation of DMEM (dulbecco′s modified eagle′s medium)/F-12 media to culture human epidermal growth factor receptor 2 (HER2) cells derived from MMTV-HER2 transgenic mouse mammary tumors
to administer the cultures to study its effect on the endogenous phosphatidyl ethanolamine pool and autophagy process
in a study as a reagent to study molecular interactions and adhesion forces in biological systems using Atomic Force Microscopy
to prepare the reagent for surface-enhanced laser desorption ionization analysis, aimed at studying the role of Interleukins

Biochem/physiol Actions

Ethanolamine hydrochloride is utilized as a carbon and nitrogen source by bacteria that differs phylogenetically. It exists as phosphotidylethanolamine in the bacterial and mammalian cell membrane. Ethanolamine-ammonia lyase is responsible for the degradation of ethanolamine into acetaldehyde and ammonia. Ethanolamine is known to positively support lipid accumulation in photosynthetic organism model.

Features and Benefits

Suitable for Immunology, Cellular Biology and Biochemical Research
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Effective Buffering Agent with a pKa of 9.5 (25 °C)