Ethidium bromide(EB) (C09-0868-050)

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.Ethidium bromide is an aromatic cationic dye, is one of the most studied DNA-intercalating agents and has been used widely as a molecular probe. It is also used in the prophylaxis and treatment of animal trypanosomiasis in affected areas worldwide. Specification: ≥95% (HPLC) MDL Number: MFCD00011724 Molecular Formula: C21H20BrN3 Molecular Weight: 394.32 PubChem CID: 14710 Isomeric SMILES: CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-] Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/E109020-SCI_826c7c5b5cba382adedeafb495693b77.pdf