Ethidium homodimer-i (ethidium bromide dimer I, ethd-i)

Product introduction：Ethidium homodimer-i (ethd-i) is a fluorescent nucleic acid dye with high affinity. After binding with DNA or RNA, it can enhance the fluorescence by more than 30 times. Used for staining of mammals, bacteria, yeast and fungi. Ethd-i has a strong positive charge, so the dye cannot cross the cell membrane for live cell staining, but it can cross the disordered region of the dead cell membrane to reach the nucleus and embed the DNA double strand to produce red fluorescence. Therefore, ethd-i can accurately detect nucleic acids in solution or in disintegrated cells, and is a more sensitive nucleic acid stain. Take adherent cells (coverslips) as an example, 100 for each sample μ L of dyeing working solution with a concentration of 4 μ M calculation, 1 mg of working fluid can be used for 2917 samples.Product parameters：Appearance: red solid soluble in DMSO and MeOH ex/em (bound DNA) = 528/617 nmcas No.: 61926-22-5 molecular formula: c46h50cl4n8 molecular weight: 856.8Matters needing attention：1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching. 3. for your safety and health, please wear experimental clothes and disposable gloves.Scope of application：Nuclear staining.