Preparation Note
Acetonitrile / 5-(Ethylthio)-1H-Tetrazole 100 / 3 (v/w)
Other Notes
Activator solution, containing 5-(Ethylthio)-1H- tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.
type: for DNA synthesis. product line: Proligo Reagents. storage condition: dry at room temperature. concentration: 32-35 . g/L (ETT content, titration). impurities: ≤. 30 . ppm water content (Karl Fischer). color: clear. SMILES string: CCSc1nnn[nH]1. InChI: 1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7). InChI key: GONFBOIJNUKKST-UHFFFAOYSA-N. Pictograms: GHS02,GHS05,GHS07. Signal Word: Danger. Hazard Statements: H225 - H302 + H312 + H332 - H315 - H318. Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2. Storage Class Code: 3 - Flammable liquids. WGK: WGK 3. Flash Point(F): 35.6 °F. Flash Point(C): 2.0 °C.