General description
EUR-2019-nCoV Modified RdRp (formerly Discrimatory) Individual Assay is for qPCR (5′ nuclease assay) detection of SARS-CoV-2.
The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.
For additional details, please visit SigmaAldrich.com/ncov.
Features and Benefits
The forward and reverse primers in each tube come with the following features:
- DNA bases
- No modifications
- Desalt purified
- 10 nmol each
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- 6-FAM™ reporter
- BXQ (Block All Quencherâ€â€SigmaAldrich.com/bxq)
- HPLC purified
- 5nmol
Components
The product includes 1 individual tube of a 20X primer and probe pool for quantification of a SARS-CoV-2 gene. Assay included is Modified RdRp.
The forward primer, reverse primer, and probe are at final concentrations of 10, 10, and 5 μM, respectively.
Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC
Legal Information
FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
- UPC:
- 41106300
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- CHA00011B-1ML
