EX-CELL(R) 325 PF CHO Serum-Free Medium for CHO Cells (C15-1301-643)

General description

EX-CELL^® 325 PF CHO is a protein-free medium which has been developed for the growth of Chinese Hamster Ovary (CHO) cells. Because it contains no large macromolecules, EX-CELL 325 PF CHO facilitates the isolation and purification of secreted proteins from the cells. CHO cells propagated in EX-CELL 302 serum-free media can be transferred directly into this protein-free medium without extensive weaning protocols. This medium is supplied without L-glutamine and does not contain purines or pyrimidines to provide an appropriate medium for specialized CHO cell lines (i.e., Glutamine Synthetase, or the GS System^™ and DHFR^- selection systems).

Quantity

Formulated to contain 21.0 grams of powder per liter of medium.

Reconstitution

Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

GS System is a trademark of Lonza Group Ltd.

description: for research or for further manufacturing use, without L-glutamine. Quality Level: 500. form: dry powder. technique(s): cell culture | mammalian: suitable. storage temp.: 2-8°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.