Application
EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or serum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
Legal Information
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
- UPC:
- 41106504
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 14420C-1000ML
